Article 5220

Title of the article

REPRODUCTIVE POTENTIAL OF THE KARELIN’S NEWT, TRITURUS KARELINII (AMPHIBIA, CAUDATA,
SALAMANDRIDAE) FROM DAGESTAN POPULATION IN LABORATORY CONDITIONS 

Authors

Kidov Artem Aleksandrovich, Candidate of biological sciences, associate professor, sub-department of zoology, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49 Timiryazevskaya street, Moscow, Russia), E-mail: kidov_a@mail.ru
Shimanskaya Elizaveta Aleksandrovna, Master’s degree student, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49 Timiryazevskaya street, Moscow, Russia), E-mail: kara-59@mail.ru
Kidova Elena Aleksandrovna, Engineer, sub-department of zoology, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49 Timiryazevskaya street, Moscow, Russia), E-mail: nemyko_e@mail.ru
Trofimets Aleksey Viktorovich, Master’s degree student, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49 Timiryazevskaya street, Moscow, Russia), E-mail: lego627403@yandex.ru
Askenderov Azim Daniyalovich, Candidate of biological sciences, senior lecturer, sub-department of zoology and physiology, Dagestan State University (43 A M. Gadzhieva street, Makhachkala, Russia), E-mail: askenderov@mail.ru 

Index UDK

591.16:597.8 

DOI

10.21685/2307-9150-2020-2-5 

Abstract

Background. The Karelin’s newt, Triturus karelinii (Strauch, 1870) – a declining species listed in the Red Data Book of Russian Federation. The North-Eastern periphery of the area is located in Dagestan. Currently, methods are being developed for captive breeding of the Karelin’s newt from the Dagestan population. The purpose of the work: to characterize the reproductive potential of the Karelin’s newt from Dagestan population, including numbers of eggs, pre-larvae and larvae obtained from each female.
Materials and methods. In 2017, 10 pairs of the Karelin’s newt were captured in the Tabasaran district of Dagestan, from which the first offspring were obtained in 2018 using hormonal stimulation. In 2019, hormone injections were not used, and offspring from newts were obtained with natural spawning. The resulting eggs and pre-larvae were kept separately in glass containers with 100 ml of water. Larvae after the start of exogenous feeding were grown in containers with 40 liters of water at an initial density of 1 individual per liter. Eggs after laying, pre-larvae, larvae and metamorphs were measured using standard methods. The duration of incubation and larval stage, as well as the survival rate were revealed.
Results. Out of ten pairs of newts, eggs were obtained from nine pairs. The first eggs were found in different females between February 28 and March 28. Females oviposited for 14–108 days. The fertility of females for the entire season was 7–615 eggs. The last cases of egg laying in different females were recorded from May 11 to June 16. The length of the eggs was 3,6–6,1 mm, and the width was 2,0–5,1 mm. From the nine breeding females, only in offspring of seven females developed embryos were observed. From egg laying to the exit of pre-larvae, 7–17 days passed, from the exit to the beginning of exogenous feeding – 2–8 days. The total duration of embryogenesis was 11–23 days. From each female, 1–97 pre-larvae and 1–93 larvae were obtained. The percentage of exited pre-larvae from eggs in different offspring was 5,0–18,8 %. 66,7–100,0 % survived from hatching to the beginning of exogenous feeding. The total length (TL) of the pre-larvae at the exit from the egg was 7,4–13,0 mm, and the larvae at the beginning of exogenous feeding was 7,6–15,7 mm. The duration of development to metamorphosis from the beginning of exogenous feeding was 98–175 days, from hatching – 100–180 days, from egg laying – 116–195 days. 224 young newts from the offspring of seven pairs the metamorphosis were passed. The survival rate to metamorphosis in young newts from all eggs laid by seven females was 12,2 %, from exit of pre-larvae from eggs – 79,7 %, from the beginning of exogenous feeding – 85,2 %. Young newts after metamorphosis had a body length (L) of 39,6–56,5 mm, a tail length of 21,1–33,4 mm, and a mass of 0,46–1,29 g.
Conclusions. Not all female of the Karelin’s newt reproduce in the laboratory every year. Eggs clutches of some females did not develop. In captivity there is an increase in female fertility and longevity of reproductive season. Due to the high embryonic mortality rate, only 1 to 97 pre-larvae were obtained from a single female. From the moment of exit the egg to metamorphosis, young newts have a very high survival rate. The main cause of death in larvae is cannibalism. 

Key words

the Karelin’s newt, Triturus karelinii, captive breeding, reproductive biology, survival, Daghestan, zooculture 

 

 Download PDF

References

1. Ananjeva N. B., Orlov N. L., Uteshev V. K., Gakhova E. N. Biology Bulletin. 2015, vol. 42, no. 5, pp. 432–439.
2. Ananjeva N. B., Uteshev V. K., Orlov N. L., Ryabov S. A., Gakhova E. N., Kaurova S. A., Kramarova L. I., Shishova N. V., Browne R. K. Russian Journal of Herpetology. 2017, vol. 24, no. 4, pp. 275–290. [In Russian]
3. Goncharov B. F., Serbinova I. A., Uteshev V. K., Shubravyy O. I. Problemy domestikatsii amfibiy: sb. nauch. tr. [Problems of domestication of amphibians: collected works]. Moscow, 1989, pp. 197–201. [In Russian]
4. Serbinova I. A., Tuniev B. S., Uteshev V. K., Shubravyy O. I., Goncharov B. F. Zookul'tura amfibiy: sb. nauch. tr. [Amphibian zoo culture: collected works]. Moscow: IEMEZh, 1990, pp. 75–81. [In Russian]
5. Shubravy O. I., Uteshev V. K., Serbinova I. A., Goncharov B. F. Amphibienforschung und Vivarium [Amphibian research and vivarium]. 1991, pp. 20–21.
6. Serbinova I. A., Tuniev B. S. I Vsesoyuznoe soveshchanie po problemam zookul'tury: tez. dokl.: v 3 ch. [The I All-Union Conference on Zooculture Problems: report thesises: in 3 parts]. Moscow, 1986, part 2, pp. 147–150. [In Russian]
7. Serbinova I. A., Shubravyy O. I., Uteshev V. K., Agasyan A. L., Goncharov B. F. Zookul'tura amfibiy: sb. nauch. tr. [Amphibian zoo culture: collected works]. Moscow, 1990, pp. 82–89. [In Russian]
8. Kidov A. A., Matushkina K. A., Afrin K. A. Vestnik Buryatskogo gosudarstvennogo universiteta [Bulletin of Buryat State University]. 2015, no. S4, pp. 81–89. [In Russian]
9. Goncharov B. F., Shubravy O. I., Serbinova I. A., Uteshev V. K. International Zoo Yearbook. 1989, vol. 28, pp. 10–21.
10. Serbinova I. A. Nauchnye issledovaniya v zoologicheskikh parkakh [Scientific research in zoological parks]. 2007, iss. 22, pp. 113–117. [In Russian]
11. Mazanaeva L. F., Kidov A. A., Dzhamirzoev G. S., Askenderov A. D., Nemyko E. A., Shimanskaya E. A. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Estestvennye nauki [University proceedings. Volga region. Natural sciences]. 2019, no. 1 (25), pp. 102–112. DOI 10.21685/2307-9150-2019-1-11. [In Russian]
12. Tuniev B. S., Lotiev K. Yu., Tuniev S. B., Gabaev V. N., Kidov A. A. Nature Conservation Research. Zapovednaya nauka [Nature Conservation Research. Nature reserve studies]. 2017, vol. 2, no. 2, pp. 1–23. DOI 10.24189/ncr.2017.002. [In Russian]
13. Tuniev B. S. Krasnaya kniga Rossiyskoy Federatsii [The Red Book of the Russian Federation]. Moscow: Astrel', 2001, pp. 312–314. [In Russian]
14. Kukushkin O. V. Krasnaya kniga Respubliki Krym. Zhivotnye [The Red Book of the Crimea. Animals]. Simferopol: IT «ARIAL», 2015, pp. 286. [In Russian]
15. Tuniev B. S., Ostrovskikh S. V. Krasnaya kniga Krasnodarskogo kraya. Zhivotnye [The Red Book of Krasnodar region. Animals]. Krasnodar: Administratsiya Krasnodarskogo kraya, 2017, pp. 478–479. [In Russian]
16. Tuniev B. S., Ostrovskikh S. V. Krasnaya kniga Respubliki Adygeya: Redkie i nakhodyashchiesya pod ugrozoy ischeznoveniya vidy zhivotnogo i rastitel'nogo mira: v 2 ch. Ch. 2: Zhivotnye [The Red Book of the Republic of Adygea: Rare and endangered species of fauna and flora: in 2 parts. Part 2: Animals]. Maykop: Kachestvo, 2012, p. 228. [In Russian]
17. Mazanaeva L. F., Askenderov A. D. Krasnaya kniga Respubliki Dagestan [The Red Book of the Republic of Dagestan]. Makhachkala, 2009, pp. 373, 374. [In Russian]
18. Askenderov A. D. Zemnovodnye Dagestana: rasprostranenie, ekologiya, okhrana: dis. kand. biol. nauk [Amphibians of Dagestan: distribution, ecology, protection: dissertation to apply for the degree of the candidate of biological sciences]. Makhachkala, 2017, 223 p. [In Russian]
19. Kidov A. A., Shimanskaya E. A., Askenderov A. D., Nemyko E. A. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Estestvennye nauki [University proceedings. Volga region. Natural sciences]. 2019, no. 4 (28), pp. 50–60. DOI 10.21685/2307-9150-2019-4-5. [In Russian]
20. Kidov A. A., Matushkina K. A., Afrin K. A. Vestnik Sankt-Peterburgskogo universiteta. Ser. 3, Biologiya [Bulletin of Saint-Petersburg University. Series 3, Biology]. 2016, no. 3, pp. 54–57. DOI 10.21638/11701/spbu03.2016.310. [In Russian]
21. Kidov A. A., Matushkina K. A., Shimanskaya E. A., Tsar'kova T. N., Nemyko E. A. Vestnik Chuvashskogo gosudarstvennogo pedagogicheskogo universiteta imeni I. Ya. Yakovleva [Bulletin of I. Yakovlev Chuvash State Pedagogical University]. 2017, no. 3 (95), pp. 10–17. [In Russian]
22. Kidov A. A., Nemyko E. A., Shimanskaya E. A. Vestnik Tverskogo gosudarstvennogo universiteta. Ser.: Biologiya i ekologiya [Bulletin of Tver State University. Series: Biology and ecology]. 2018, no. 4, pp. 38–49. DOI 10.26456/vtbio26. [In Russian]
23. Litvinchuk S. N., Borkin L. Ya. Evolyutsiya, sistematika i rasprostranenie grebenchatykh tritonov (Triturus cristatus complex) na territorii Rossii i sopredel'nykh stran [The evolution, systematics, and distribution of comb tritons (Triturus cristatus complex) in Russia and neighboring countries]. Saint-Petersburg: Evropeyskiy dom, 2009, 592 p. [In Russian]
24. Kuz'min S. L. Zemnovodnye byvshego SSSR [Amphibians of the former USSR]. Moscow: Tovarishchestvo nauchnykh izdaniy KMK, 2012, 370 p. [In Russian]
25. Kidov A. A., Matushkina K. A. Aktual'nye problemy ekologii i sokhraneniya bioraznoobraziya Rossii i sopredel'nykh stran: sb. nauch. tr. [Actual problems of ecology and conservation of biodiversity of Russia and neighboring countries: collected works]. Vladikavkaz: Izd-vo SOGU im. K. L. Khetagurova, 2012, iss. 8, pp. 46–50. [In Russian] 

 

Дата создания: 14.07.2020 09:47
Дата обновления: 22.07.2020 09:46